Canine Parvovirus Research
Molecular Characterization and Phylogenetic Analysis of CPV-2 Variants
Project Overview
This comprehensive research project focuses on the molecular characterization, epidemiology, and evolutionary analysis of Canine Parvovirus Type 2 (CPV-2) variants. Through advanced bioinformatics approaches and molecular techniques, we investigated the genetic diversity, mutation patterns, and phylogenetic relationships of CPV-2 strains circulating in different geographic regions.
Canine Parvovirus is a highly contagious viral disease affecting dogs worldwide, causing severe gastroenteritis and myocarditis. Understanding the molecular evolution and genetic variations of CPV-2 is crucial for developing effective vaccination strategies and disease management protocols. Our work contributes to the global understanding of parvovirus evolution and host adaptation mechanisms.
Project Details
- Status: Published
- Duration: 2022-2023
- Role: Bioinformatics Researcher
- Field: Molecular Virology
- Journal: Acta Tropica
Research Objectives
Molecular Characterization
Identify and characterize genetic variants of CPV-2 through sequence analysis and mutation mapping.
Phylogenetic Analysis
Construct phylogenetic trees to understand evolutionary relationships and geographic distribution patterns.
Epidemiological Insights
Provide data-driven insights for disease surveillance and vaccine development strategies.
Methodology
Bioinformatics Approaches
- Sequence alignment and comparative genomics
- Multiple sequence alignment (MSA) analysis
- Phylogenetic tree construction using ML methods
- Selection pressure analysis (dN/dS ratios)
- Structural variant identification
Data Analysis
- NCBI GenBank database mining
- Statistical analysis of mutations
- Geographic distribution mapping
- Temporal evolution tracking
- Protein structure prediction and modeling
Key Findings & Contributions
- Identified novel CPV-2 variants with unique amino acid substitutions in the VP2 capsid protein
- Characterized mutation hotspots associated with host adaptation and antigenic variation
- Constructed comprehensive phylogenetic trees revealing evolutionary pathways and geographic clustering
- Analyzed selection pressures on key viral genes to understand evolutionary dynamics
- Provided epidemiological insights for veterinary public health and disease control strategies
- Published findings in Acta Tropica, contributing to the global veterinary virology knowledge base
Technology Stack
Impact & Results
200+
Sequences Analyzed
10+
Countries Covered
Novel
Variants Identified
Published
in Acta Tropica
Research Applications
🐕 Veterinary Medicine
Inform vaccine development and update strategies based on circulating viral variants.
🦠 Disease Surveillance
Support epidemiological monitoring and early detection of emerging variants.
🔬 Evolutionary Biology
Advance understanding of viral evolution, host adaptation, and cross-species transmission.
Interested in Molecular Virology Research?
Let's discuss viral genomics, phylogenetics, or bioinformatics collaborations.